Optimum conditions for electric pulse-mediated gene transfer to mammalian cells in suspension.

نویسندگان

  • H Hama-Inaba
  • M Takahashi
  • M Kasai
  • T Shiomi
  • A Ito
  • F Hanaoka
  • K Sato
چکیده

A pulse-generating machine which delivers exponentially decaying pulses over broad range of pulse lengths was used to determine the optimum pulse conditions for gene transfer to FM3A cells. In the transformation of tk- cells with pTK1, a single pulse of 100-2000 microseconds gave a high transformation frequency at 1.5-6 kV/cm and room temperature, the highest transformation frequency obtained being 3 X 10(-3). As the suspension buffer for cells exposed to the pulse, Saline G was better than PBS(-) for obtaining a large number of transformants because it ensured high cell viability.

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عنوان ژورنال:
  • Cell structure and function

دوره 12 2  شماره 

صفحات  -

تاریخ انتشار 1987